http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3610039-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6f41a868f49ca4f32d18d861e3afb411 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-191 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-1025 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2533-107 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6841 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6841 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-682 |
| filingDate | 2018-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68686ebcd0b34b5b032caf0e89491d05 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_47406bff68f485c8565395e287bfd6a6 |
| publicationDate | 2020-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3610039-A1 |
| titleOfInvention | Labeling of oligonucleotide probes by multiple-way ligation |
| abstract | The present invention provides a novel method for multiple labelling of nucleic acid probes by ligation. The method uses a ligase catalysed reaction to connect a nucleic acid probe with multiple pre-prepared nucleic acid label carrier molecules under the presence of a stabilizing complementary adaptor oligonucleotide. The method allows for an easy, cheap and fast labelling of multiple probes with multiple different labels. In this way, the costs and effort for the generation of single molecule Fluorescent In Situ Hybridization (smFISH) assays was significantly reduced, allowing combinatorial multi-color barcoding of nucleic acid probes The invention further provides methods for the generation of FISH libraries and labelling kits comprising the novel tools of the invention. |
| priorityDate | 2017-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 92.