| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5a63903517aecd02ac5ee234ec9d5dea |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-191
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-122 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate |
2018-03-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3d36ea96347f343a2df922dc94b57d2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_345bfa8cff6fe397ebbaa607ef36aa6a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_648f510309754eceffc0ac7d93a85648
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_943fe94cf1fd139a3ca46eeb14b9b92a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_667ad4a3d5a522f2c7b58f767848d354
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9990d18d992ad76d80f36e7c6160bc88 |
| publicationDate |
2020-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3608452-A1 |
| titleOfInvention |
Method for constructing amplicon library through one-step process |
| abstract |
The present invention discloses a method for rapidly constructing amplicon library through one-step process, comprising the following steps: 1. Synthesizing a primer combination for constructing an amplicon library of a DNA sample, the primer combination of the amplicon library used to construct the DNA sample includes: an upstream fusion primer designed according to the target amplicon, a downstream fusion primer designed according to the target amplicon, an upstream universal primer and a downstream universal primer; 2. Constructing a PCR reaction system for the DNA sample; 3. Performing PCR. This method according to the present invention can be used to construct an amplicon library in a simple and rapid manner, and since the barcode is introduced before the start of PCR, the possibility of cross-contamination between the sample and the library is greatly reduced. |
| priorityDate |
2017-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |