http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3608411-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c3d10bbb7c0b69ddc83b452380f907d9 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8278 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8275 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8274 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-821 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8277 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 |
| filingDate | 2018-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb5065da1eeb12e88ad0da284eeede6a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_96619b75805766471a81eadca89a851c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4175ab9b23b798e17b8be91f711381f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a64d15d5bac3a9a2f4bd9f9d4cec544f |
| publicationDate | 2020-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3608411-A1 |
| titleOfInvention | Method for improving transformation efficiency of soybean |
| abstract | The present invention relates to a method of improving soybean transformation efficiency, which comprises: transforming a plant cell using a recombinant vector containing a gene of interest and a gene encoding a sulfonylurea herbicide hydrolase; screening and culturing the transformed plant cell by external application of an ALS inhibitor, using the gene encoding the sulfonylurea herbicide hydrolase as a selective marker; selecting a plant cell that has not been killed and/or not been inhibited. The present invention firstly proposes that a selective agent is added to a proliferation medium and a differentiation medium in a manner of external application during plant transformation process, and optimizes the effective screening concentration range of the selective agent, so the transformation efficiency is remarkably increased, and the proportion of positive plants obtained in the progeny thereof is significantly increased; at the same time, the transgenic plants obtained by the transformation using the sulfonylurea herbicide hydrolase gene as a selective marker in the present invention have high commercial value, good resistance and genetic stability. |
| priorityDate | 2017-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 926.