| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e67739af533921705c6fdeb7b3b25efe |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y605-01001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-02027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07007 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1037
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1072
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B20-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B30-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2017-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc141320c29cd184c8bc78f047842821
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f5a1e43cf3317f6ce2352533e20fdd9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4e68426d97f11505cdbb4bc5ee9fa31
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b6a35023d95b1cb9cb018d24675557a2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d01d9a28e72c2bc04e0b29015cabc39
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b3ab4d1c19f357741453cae2551d2c23 |
| publicationDate |
2019-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3562941-A1 |
| titleOfInvention |
Method for generating higher order genome editing libraries |
| abstract |
The present invention pertains to a novel method for the generation of highly diverse RNA expressing vectors and vector libraries for use in targeted gene knock out, knock down and genome modification approaches. The invention pertains to a method for generating such higher order libraries without the need of classical cloning technologies. This is particularly useful for libraries based on large vectors wherein a sequence cannot be easily mutated with classical mutagenesis methods. The vectors and libraries generated according to the methods of the invention are in particular for RNA assisted silencing technologies such as RNA interference, and for targeted genome editing using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system or similar RNA/DNA-encoded gene perturbation systems which use small guide RNAs to target the CRISPR complex to a specific genomic sequence. The invention provides also kits comprising the materials for performing the methods of the invention. |
| priorityDate |
2016-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |