| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0c37ea6c0ccf659261758e2450ac848e |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-05
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2513-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0663
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0696 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 |
| filingDate |
2018-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a6eba99a8d3eb48cb43afdf63444259
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06a45d10b08c1b1e4a4928313d3071bd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_583ceb82a5157d89e19bea093c326af9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e7b616bc859e7be88a5130ff562072e7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c011f1c62ffa99d254422631af199a56 |
| publicationDate |
2019-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3555264-A1 |
| titleOfInvention |
Passaging and harvesting formulation for single-cell human pluripotent stem cells |
| abstract |
The field of the invention is cellular and molecular biology and stem cells. Specifically, the disclosure is directed to a formulation for harvesting and passaging single cell human pluripotent stem cells comprising: (i) 1 mM to about 30 niM sodium citrate; (ii) a salt comprising 10 mM to 170 mM KC1 or NaCl; and (iii) Ca2+/Mg2+-free Dulbecco's phosphate buffered saline (DPBS), wherein said formulation has an osmoiarity of about 100 mOsmol/liter to about 350 mOsmol/liter. The formulation can be used for serial passaging and dislodging of pluripotent stern cells attached to 2D tissue culture vessels or grown in 3D suspension culture (small scale and large scale bioreactors) or any other application where passaging in the form of single cell population of stem cells is needed. |
| priorityDate |
2017-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |