http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3551768-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4bd24d0edc441a277cf5d128b2c97b31 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07049 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y605-01003 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6855 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 |
| filingDate | 2017-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df611ebc7c8448963f02d9a0effcd3c2 |
| publicationDate | 2019-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3551768-A1 |
| titleOfInvention | Methods for tagging and amplifying rna template molecules for preparing sequencing libraries |
| abstract | Methods for preparing sequencing libraries based on a plurality of RNA molecules which are tagged and amplified by tagging the molecule with an oligonucleotide hybridising to a polyC tail introduced by the terminal transferase activity of the reverse transcriptase, e.g. MMLV RT, and ligating the oligonucleotide to the RNA molecule using a ligase, e.g. T4 RNA ligase, and producing cDNA molecules based on mRNA and strand displacing reverse transcriptases, e.g.g MMLV RT, and producing a second cDNA strand in order to produce a dsDNA library for sequencing. In some embodiments, the subject methods comprise sequencing at least a portion of a sequencing library to obtain sequencing data or sequence reads from a test sample (e.g., a biological sample from a subject). |
| priorityDate | 2016-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.