Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c8c293fd340e0c8b6a445f7633453a0 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-5027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-0663 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-0652 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-1805 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2400-0478 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-122 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-143 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2400-0644 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0864 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0861 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-087 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-149 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-164 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2523-125 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L7-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N35-00 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate |
2017-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c5683baf7c454587d1d1ed8359dc0c6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58a7ddb684966eac1afdb89de47881e6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15f0b04d47fa2d11c5f0db5da4d2d8b7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eca5ce1a15aaba1da0e5b022e326445b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2edb5559e67b394cff5394a0a1332de6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_824eda6010da93bbfc18b801785dde0b |
publicationDate |
2019-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-3551332-A1 |
titleOfInvention |
Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge |
abstract |
Methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and/or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bi sulfite ions to produce a deaminated nucleic acid; iv) contacting said deaminated nucleic acid to a second matrix material comprising a second column to bind said deaminated nucleic acid to said second matrix material; v) desulphonating the bound deaminated nucleic acid and/or simultaneously eluting and desulphonating the nucleic acid by contacting the deaminated nucleic acid with an alkaline solution to produce a bi sulfite converted nucleic acid; vi) eluting said bi sulfite converted nucleic acid from said second matrix material; and vii) performing methylation specific PCR and/or nucleic acid sequencing, and/or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein at least steps iv) through vi) are performed in a single reaction cartridge. |
priorityDate |
2016-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |