http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3504338-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_295d4b710bb15b2b8f23349b28099215 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2017-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3e08f04ec81d82381f364a9ff009da9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8229d80ceffc3c01b133d24fb73ee82f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8bc2f24492af1d6be80be2ac7b34b8c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_31235a587ee2a87314849e45399b7fac http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d9630213e7e33d1fdcd6ff2175b3145 |
| publicationDate | 2019-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3504338-A1 |
| titleOfInvention | Process for the amplification of nucleic acids and use of a kit for carrying out the same |
| abstract | There is disclosed a process for the amplification of nucleic acids that essentially makes use of the fact that a predefined nucleic acid chain (target sequence) can be multiplied/amplified in the presence of a target-sequence-specific activator oligonucleotide. The target-sequence-specific activator oligonucleotide brings about the separation of de-novo synthesized complementary primer elongation products by means of strand displacement, so that a new primer oligonucleotide can attach to the respective strand of the template. The complex thus formed, of a primer oligonucleotide and a template strand, can initiate a new primer elongation reaction. The primer elongation products thus formed, in turn, act again as templates, the result being an exponentially proceeding amplification reaction. The activator oligonucleotide does not act as a template itself here and is preferably inert to a primer elongation reaction. The activator oligonucleotide acts as cofactor in the reaction and regenerates the single-strand state of the relevant strand segments of the templates, which is required for the reaction. By using a set of components comprising at least one primer oligonucleotide, at least one polymerase, a set of substrates for the polymerase (for example dNTPs) and at least one specific activator oligonucleotide it is possible to amplify, in a linear fashion, a nucleic acid to be amplified. By using such a set and a second primer oligonucleotide, a nucleic acid to be amplified can be amplified exponentially, in which process resulting specific primer elongation products are used as templates for further primer elongation reactions. |
| priorityDate | 2016-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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