| abstract |
A method of producing a co-culture of intestinal epithelial cells and microbial cells, comprising providing a cell culture system comprising a fluidic device having a fluid channel connected to a fluid source, the fluid source supplying fluid to the fluid channel; and a membrane positioned within the channel. The method further comprises providing a fluid suitable for maintaining intestinal epithelial cells to the cell culture system such that the fluid contacts the intestinal epithelial cells, culturing the intestinal epithelial cellswherein the intestinal epithelial cells adhere to at least one surface of the membrane, maintaining microbial cells in the system for at least 1 day, wherein the microbial cells adhere to the surface of the viable intestinal epithelial cells, and flowing fluid continuously through the channel such that microbial cells not adhering to the intestinal epithelial cells are washed out. |