| abstract |
The object of the present invention is to generalize and improve DNA-binding proteins using PPR. There is provided a protein that contains one or more PPR motifs having a structure of the following formula 1, wherein one PPR motif (M) contained in the protein is a PPR motif having a specific combination of amino acids corresponding to a target DNA base or target DNA base sequence as the three amino acids of No. 1 A.A., No. 4 A.A., and No. "ii" (-2) A.A, and satisfies at least one selected from the group consisting of the following conditions (a) to (h): (a) No. 7 A.A. of the PPR motif (M) is isoleucine (I); (b) No. 9A.A. of the PPR motif (M) is alanine (A); (c) No. 10 A.A. of the PPR motif (M) is tyrosine (Y); (d) No. 18 A.A. of the PPR motif (M) is lysine (K), arginine (R), or histidine (H); (e) No. 20 A.A. of the PPR motif (M) is glutamic acid (E), or aspartic acid (D); (f) No. 29 A.A. of the PPR motif (M) is glutamic acid (E), or aspartic acid (D); (g) No. 31 A.A. of the PPR motif (M) is isoleucine (I); and (h) No. 32 A.A. of the PPR motif (M) is lysine (K), arginine (R), or histidine (H). |