| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_734ae3e52ec22e67a9358a41c51ff1dc |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-31
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-7019
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-811
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-325
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-545 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6893
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-163
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-535
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6887
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-543 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-26 |
| filingDate |
2003-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04a2f77aeadd74571940f753a4c9ba88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e15ec45648223659a65e7b893745578f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ed06d8f2114136f55fa2cc92e3b96fff
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3df3f0fd73217c1021c86bc14e1daf7 |
| publicationDate |
2019-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3450457-A1 |
| titleOfInvention |
Polyclonal-monoclonal elisa assay for detecting n-terminus probnp |
| abstract |
A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure circulating NT-proBNP in human plasma. In order to obtain antibodies with specific binding properties for targeted amino acid sequences within human proBNP, recombinant human proBNP (or rhproBNP) was expressed and purified for use as an immunogen. Polyclonal antibodies (PAb) to specific amino acid sequences were subsequently purified from goat serum by sequential affinity purification. Monoclonal antibodies were raised against specific polypeptides; Recombinant human NT-proBNP (or rhNT-proBNP) was expressed and purified in order to obtain material for use in calibration of a quantitative method for measurement of human NT-proBNP. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11016106-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111848775-A |
| priorityDate |
2002-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |