| abstract |
Provides an ex vivo expansion method for a cord blood NK cell, and a kit and application thereof. The ex vivo expansion method for the cord blood NK cell comprises: separating a cord blood monocyte; activating, culturing, and proliferation; and culturing of the cord blood NK cell. The invention does not require cell sorting and does not require a trophoblast. A reagent in the kit does not contain animal-based component. The invention provides a high production volume of the NK cell. The cord blood NK cell obtained has at least 90% purity, a total cell count of up to 1010-11 per part of cord blood, and can kill a tumor cell effectively. |