| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_36b8402faef868b9530993bab6e10e6e |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-55
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2320-51
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2330-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-3181 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C229-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07F9-222
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C229-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C229-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C229-34 |
| filingDate |
2017-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f0a48dc2b3d5a63173a5d97d154cce1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aac1faac68bb1094ed8a66f06275e321
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5587cfe0c798902df493a86e10e986d |
| publicationDate |
2018-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3409776-A1 |
| titleOfInvention |
Artificial single guide rna and use thereof |
| abstract |
The present invention provides a novel artificial sgRNA having an activity equal to or higher than that of a natural form sgRNA and improved in stability in vivo, and an efficient CRISPR/Cas9 system by combining the artificial sgRNA and Cas9. Even when a nucleotide linker region for forming a single strand by linking the 3'-terminal of crRNA and the 5'-terminal of tracrRNA in sgRNA is substituted with an amino acid derivative linker, an activity equal to or higher than that of the original sgRNA is retained in an in vitro DNA cleavage assay. Furthermore, even when the linker region existing between stem-loop 1 and stem-loop 2 of tracrRNA and/or the loop portion of stem-loop 2 are/is substituted with an amino acid derivative linker, or even when an amino acid derivative linker is added/inserted into the vicinity of the 5'-terminal and/or the 3'-terminal of sgRNA, an activity equal to or higher than that of the original sgRNA is retained. The stability in vivo can be improved by introducing one or more amino acid derivative linkers into sgRNA. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023081687-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3823633-A4 |
| priorityDate |
2016-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |