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filingDate 2016-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2018-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-3373944-A1
titleOfInvention A method for producing a medium containing steroidal glycosides from plant cells of the genus hoodia
abstract The invention provides for a method for producing an active ingredient-containing medium from cultured plant cells, selected from the Hoodia genus. The method comprises the steps of: (i) incubating a mixture of cultured plant cells of the genus Hoodia and a liquid media in light; (ii) separating the incubated mixture into a first portion and a second portion; (iii) passing the first portion through a tangential flow filter to produce a filtrate containing only media and a retentate containing cultured plant cells; (iv) passing the filtrate through a nano-filter to obtain a nano-retentate having a high concentration of steroidal glycosides; (v) reintroducing some of the nano-retentate into the second portion; and (vi) repeating steps (i) to (v) using the second portion. The medium may be either plant cells or a plant extract. The method is advantageous as it can ensure that the plant cells are cultured in a media containing high levels of steroidal glycosides, thereby maximising the amount of steroidal glycosides in both the media and the plant cells.
priorityDate 2015-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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