Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_49310188df8dc04b3708491df863459f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2a819eda0adf22936a52362eeebb9fb4 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-0652 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-143 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-0668 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2400-086 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0681 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502753 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1017 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-08 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M3-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M3-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B03C5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2016-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_71c90a206452c0ef1536653005dbcd30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e31854c81c676b666dcdd17971dd453 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4401d6561f19691e3794ad0fb0780cff http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7142af3e92a94b3b5ffd1f7c68a27624 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3228f57da80c23d1274c3b3270af2669 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f7b2d4cf2f6a4598ea4bff4ce066b96b |
publicationDate |
2018-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-3362179-A1 |
titleOfInvention |
Single cell microfluidic device |
abstract |
Methods of non-destructively obtaining the genotype of a plant cell from a plant sample are disclosed. The plant cell is isolated from a plant cell sample using an integrated microfluidic device. The integrated microfluidic device includes an individual cell trap located downstream from a microfluidic channel and, the microfluidic device is configured to trap the plant cell in the individual cell trap. mRNA is extracted from the plant cell by contacting the plant cell with an atomic force microscope (AFM) probe and by attracting mRNA from loci of interest to the probe end using a dielectrophoresis DEP force under the alternating current (AC) field applied to the probe. The genotype of the plant cell is determined from cDNA obtained from the extracted mRNA. Alternatively, the mRNA is analyzed to determine gene expression patterns of the plant cell. |
priorityDate |
2015-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |