| abstract |
The present disclosure provides variant Cas9 proteins (e.g., reporter Cas9 proteins), nucleic acids encoding the variant Cas9 proteins, and host cells comprising the nucleic acids. The present disclosure provides systems and kits that include a subject variant Cas9 protein (e.g., reporter Cas9 proteins) (and/or a nucleic acid encoding the variant Cas9 protein). The variant Cas9 proteins (e.g., reporter Cas9 proteins) and the nucleic acids encoding the variant Cas9 proteins are useful in a wide variety of methods (including the detection of a conformational change of the variant Cas9 protein), which are also provided. |