abstract |
Device and process for the automated extraction of nucleic acids, comprising a body which is able to be immersed partly or wholly into a reaction cavity, characterized in that at least the part immersed into the reaction cavity has a non-smooth surface. After lysis, a biological sample is admixed with an organic substance, preferably alcohols or ketones. This batch is then contacted with a material characterized by a non-smooth surface. In these circumstances, nucleic acids are adsorbed onto the surface of the material used. This is followed optionally by washing steps with known alcoholic washing solutions. After drying, the adsorbed nucleic acid is detached from the material by adding water or a low-salt buffer and can be used for downstream applications. |