| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5098eff6d67391cd857fae0aa3174bcb |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-92 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-44
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01003 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2016-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3841afb2cac8e042f3509060b6f78cea
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3576c71f5182910c0a64916af00ffd12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03782c7f36968d3a5b93189b324d2ab5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_baa9dd231668785ab463f0bf933a00d1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3abdf36a0e3bb7cbe7d0c8978262fa06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a505e32d2e6ea107ce4136115cf1ae2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_187e4799e0983719f755f61aa570e0bd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0968eefadabef2967baa707210e00d27 |
| publicationDate |
2018-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3280818-A2 |
| titleOfInvention |
Methods for selecting enzymes having lipase activity |
| abstract |
Provided herein are methods and means for enhancing lipase activity. The system makes use of an emulsion for in vitro compartmentalization of a library of synthetic compounds which have a polynucleotide linked to a lipase substrate (e.g., a triglyceride). Expressed polypeptides having greater lipase activity will preferentially hydrolyze the substrate from the linked polynucleotide. Genes encoding polypeptides having less lipase activity will remain linked to the substrate and may be removed to enrich the library for more active variants. Also described are synthetic compounds and emulsions which can be used in the methods. |
| priorityDate |
2015-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |