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filingDate 2016-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_97ac352c729cc2b44185dd0c96f230fa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b2bf7e67bcdcb1e60efe1687cf753e0c
publicationDate 2018-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-3274721-A1
titleOfInvention Retention time standard
abstract The invention relates to a composition as well as a polypeptide comprising at least 8 reference peptides (R-peptides) selected from the group consisting of SEQ ID NO.: 1 - 27 and variants thereof, wherein at least one R-peptide is selected from each group comprising group 2 consisting of SEQ ID NO.: 3 - 5 and variants thereof, group 3 consisting of SEQ ID NO.: 6 - 8 and variants thereof, group 4 consisting of SEQ ID NO.: 9 - 11 and variants thereof, group 5 consisting of SEQ ID NO.: 12 - 18 and variants thereof, and group 6 consisting of SEQ ID NO.: 19 - 23 and variants thereof, wherein each variant differs from the original peptide in 30 % or less of the amino acids. The invention further relates to the use of the at least 8 reference peptides for reverse-phase liquid chromatography and selected reaction monitoring. Furthermore, the invention relates to a method for monitoring reverse-phase chromatography, comprising the steps providing the at least 8 reference peptides, applying the R-peptides to a chromatography column, and determining the retention time of each peptide. Furthermore, the invention relates to a method for estimating a retention time of a target peptide during a liquid chromatography run.
priorityDate 2015-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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