http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3273238-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6d9006a67809c9e565440e678672f777 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-42 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-564 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-564 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 2016-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b0e05abb99dd82fb65c316e45104ff5 |
| publicationDate | 2018-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3273238-A1 |
| titleOfInvention | Immunodiagnostic detection of anti-neutrophil antibodies |
| abstract | The invention relates to immunodiagnostic detection of anti-neutrophil antibodies. Object of the current invention is to improve the diagnosis of ANCA-associated diseases, in particular ANCA-associated vasculitides. For solving the problem, the invention provides in one aspect a method for the immunodiagnostic detection of anti-neutrophil antibodies, the method comprising: na1) Contacting an aliquot of a sample of body fluid with stimulated netotic but not netting polymorphonuclear neutrophils, PMNs, under conditions allowing binding of anti-neutrophil antibodies in the sample to the polymorphonuclear neutrophils, wherein the netotic but not netting polymorphonuclear neutrophils have been generated by treating stimulated, but not netting, polymorphonuclear neutrophils with a NET release inhibiting compound under conditions where the formation of NETs by the stimulated polymorphonuclear neutrophils is suppressed, and na2) Immunolabeling of anti-neutrophil antibodies bound to the stimulated netotic but not netting polymorphonuclear neutrophils. n In further aspects the invention provides a method for preparing an object slide for the immunodiagnostic detection of anti-neutrophil antibodies, an object slide for the immunodiagnostic detection of anti-neutrophil antibodies, a kit for the immunodiagnostic detection of anti-neutrophil antibodies, and the use of stimulated neutrophils for the immunodiagnostic detection of anti-neutrophil antibodies. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11085922-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3499235-A1 |
| priorityDate | 2016-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 118.