http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3265808-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_42a59c5faed1d0699d86edc5a2eee671 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B32B43-006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B32B38-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T156-1994 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T156-1944 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T156-1132 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T156-1174 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T156-195 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T156-1195 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02F1-1335 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A47L9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0619 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02B5-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09J7-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5058 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 2016-03-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1e1fc6ef02ee7552fa22407d92a3ef1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_071498bf4688f9ac731c3f23173f1774 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a59af3f9873d5d712170e0524cdc1d70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c34e5c051c772f6e4c942193ef1cff2e |
| publicationDate | 2018-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3265808-A1 |
| titleOfInvention | Methods for enhancing the specific uptake of botulinum neurotoxins into cells |
| abstract | The present invention provides a method for enhancing the specific uptake of a neurotoxin polypeptide into cells, the method comprising: incubating cells susceptible to neurotoxin intoxication with a neurotoxin polypeptide for a time and under conditions which allow for the neurotoxin polypeptide to exert its biological activity, the incubation comprising at least one of the following steps: (i) K + -mediated depolarization of the cells, (ii) a reduced neurotoxin polypeptide exposition time and/or (iii) agitation of the cells during neurotoxin polypeptide exposition, thereby enhancing the specific uptake of the neurotoxin polypeptide into said cells. In addition, the invention pertains to a method for directly determining the biological activity of a neurotoxin polypeptide in cells, comprising: a) incubating cells susceptible to neurotoxin intoxication with a neurotoxin polypeptide for a time and under conditions which allow for the neurotoxin polypeptide to exert its biological activity, the incubation comprising at least one of the following steps: (i) K + -mediated depolarization of the cells, (ii) a reduced neurotoxin polypeptide exposition time and/or (iii) agitation of the cells during neurotoxin polypeptide exposition; b) fixing the cells and, optionally, permeabilizing the cells with a detergent; c) contacting the cells with at least a first capture antibody specifically binding to the non-cleaved and neurotoxin-cleaved substrate and with at least a second capture antibody specifically binding to the cleavage site of the neurotoxin- cleaved substrate, under conditions which allow for binding of said capture antibodies to said substrates; d) contacting the cells with at least a first detection antibody specifically binding to the first capture antibody, under conditions which allow for binding of said first detection antibody to said first capture antibody, thus forming first detection complexes and with at least a second detection antibody specifically binding to the second capture antibody, under conditions which allow for binding of said second detection antibody to said second capture antibody, thus forming second detection complexes; e) determining the amount of the first and second detection complexes of step d); and f) calculating the amount of substrate cleaved by said neurotoxin polypeptide in said cells by means of the second detection complexes, thereby determining the biological activity of said neurotoxin polypeptide in said cells. |
| priorityDate | 2015-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.