http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3257942-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_102641a882d1cb61968495c694bd7476 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2009-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_85477a717407586ce29d1097075d24e5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a29c1fbecbf803f2117ff2d1cde79755 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f85b019d0b5a7e07caa7ad17544927d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6185a27da546b4448b32f1c18c528975 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce28e3ad96b07bb675891605c2abff88 |
| publicationDate | 2017-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3257942-A1 |
| titleOfInvention | Method for assembly of polynucleic acid sequences |
| abstract | A method for the assembly of a polynucleic acid sequence from a plurality of nucleic acid sequences in which the polynucleic acid sequence is of a formula N n+1 , in which N represents a nucleic acid sequence and where n is 1 or greater than 1 and each N may be the same or a different nucleic acid sequence, in which the method comprises: (i) providing a first nucleic acid sequence N1 in a vector, using a restriction enzyme to cut the nucleic acid sequence out from the vector and ligating an oligonucleotide linker sequence L1 3' to the 3'-end of the nucleic acid sequence; (ii) providing a second nucleic acid sequence N2 in a vector, using a restriction enzyme to cut the nucleic acid sequence out from the vector and ligating an oligonucleotide linker sequence L2 5' to the 5'-end of the nucleic acid sequence, wherein the 5'-end linker sequence L2 5' of nucleic acid sequence N2 is complementary to the 3'-end linker sequence L1 3' of nucleic acid sequence N1; and ligating an oligonucleotide linker sequence L2 3' to the 3'-end of the nucleic acid sequence N2; and (iii) providing one or more additional nucleic acid sequence(s) N in one or more vector(s), using a restriction enzyme to cut the nucleic acid sequence(s) out from the vector(s), wherein nucleic acid sequence N2 has an oligonucleotide linker sequence L2 3' at the 3'-end of the nucleic acid sequence, and wherein said one or more additional nucleic acid sequences N comprises a terminal additional nucleic acid sequence NZ, and ligating an oligonucleotide linker sequence to the 3'-end of each additional nucleic acid sequence N, wherein said terminal additional nucleic acid sequence NZ optionally lacks an oligonucleotide linker sequence at its 3'-end, and ligating an oligonucleotide linker sequence to the 5'-end of each additional nucleic acid sequence N, wherein for the first additional nucleic acid sequence N3 the 5'-end linker sequence L3 5' is complementary to the 3'-end linker sequence L2 3' of nucleic acid sequence N2 and for each second and subsequent additional nucleic acid sequence N the 5'-end linker sequence is complementary to the 3'-end linker sequence of the respective preceding additional nucleic acid sequence. |
| priorityDate | 2008-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 169.