http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3246706-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f97f0fa258a008bf056ad04b7f380dda http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7e5249117b2e40524eb0863677e8be2a |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C205-59 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6842 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/H01J49-0059 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C205-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-128 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/H01J49-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2017-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e44f62e49ee35e36fc1d1849eb017cfc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0bd527544cfbf55836d6686aaee137ce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0aa840f2353f0efd1269e8da7407493e |
| publicationDate | 2017-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3246706-A1 |
| titleOfInvention | Method for specific cleavage of c(alpha)-c bond and side chain of protein and peptide, and method for determining amino acid sequence |
| abstract | The present invention provides a method for specifically cleaving a Cα-C bond of a peptide backbone and/or a side chain of a protein and a peptide, and a method for determining amino acid sequences of protein and peptide. A method for specifically cleaving a Cα-C bond of a peptide backbone and/or a side chain bond of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of at least one hydroxynitrobenzoic acid selected from the group consisting of 3-hydroxy-2-nitrobenzoic acid, 4-hydroxy-3-nitrobenzoic acid, 5-hydroxy-2-nitrobenzoic acid, 3-hydroxy-5-nitrobenzoic acid, and 4-hydroxy-2-nitrobenzoic acid. A method for determining an amino acid sequence of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of the above specific hydroxynitrobenzoic acid to specifically cleave a Cα-C bond of a peptide backbone and/or a side chain bond, and analyzing generated fragment ions by mass spectrometry. |
| priorityDate | 2016-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 107.