http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3234176-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3f0d1964c25696c35eb233f1b895090c |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-916 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-137 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-155 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-319 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-301 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-307 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-922 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-327 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2015-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_650c016400279497b8786a0a7f63360a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc0c931bb937e1bab8a382729a4adec3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19b96f763dfa1b7e788cf6215890a650 |
| publicationDate | 2017-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3234176-A1 |
| titleOfInvention | Method for the colorimetric detection of contamination with nucleases |
| abstract | Method for detecting the presence of nucleases in a sample, characterized in that it comprises the steps of: - incubating the sample to be tested for the presence of nucleases with at least one oligonucleotide linker constituting the substrate for the nuclease to be detected, for a sufficient time to cause degradation of said oligonucleotide linker by the nuclease possibly present in the sample, - adding to the sample, upon incubation, colloidal gold nanoparticles comprising gold nanoparticles functionalized with a first probe oligonucleotide and gold nanoparticles functionalized with a respective second probe oligonucleotide, said first and second probe oligonucleotides being complementary to a respective portion of the nucleotide sequence of the oligonucleotide linker, and - examining the possible colour change of the sample as a result of the addition of said nanoparticles, a colour change of the sample to the colour assumed by the colloidal gold particles when aggregated at a distance less than their size being indicative of the absence of the tested nuclease from the sample. |
| priorityDate | 2014-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.