abstract |
A method comprising comminuting a lignocellulosic biomass material; cooling the comminuted biomass material to 280° K (6.85° C) or less; after cooling, comminuting the cooled biomass material; either before or after cooling, irradiating the biomass material; after the second comminuting step, contacting the material with an enzyme and/or a microorganism to release sugars; and fermenting the released sugars. |