| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1b5d47c4f1d77d82de7ce1f64ba985b3 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-0036
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-142
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0829
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-0636
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0627
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0636
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-0647
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00702
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00722
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-7786
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-0668
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00317 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502784
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502761
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502715
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J19-0046
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502776
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-0268
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-77 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L3-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L3-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J19-00 |
| filingDate |
2016-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
2017-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3207982-A1 |
| titleOfInvention |
Improved droplet sequencing apparatus and method |
| abstract |
A first apparatus for sequencing a polynucleotide analyte is provided and comprises; n¢ a first zone in which a stream of single nucleotide phosphates is generated by progressive pyrophosphorolysis of a molecule of the analyte attached to a particle and exposed to a flowing aqueous medium; n¢ a second zone in which a corresponding stream of aqueous droplets is generated from the aqueous medium and the nucleotide stream and wherein at least some of the droplets contain a single nucleotide triphosphate and n¢ a third zone in which each droplet is stored and optionally interrogated to reveal a property characteristic of the single nucleotide it may contain. It is characterised in that the first zone includes at least one chemically-modified substrate adapted to bind temporarily to a particle having at least two different regions adapted to bind respectively to the substrate and to the analyte. Suitably the particles comprise at least one metal coated first region(s) and at least one second region(s) comprised of reactive sites to which the analyte can be attached. In one embodiment the two regions each comprise hemispheres of a substantially spherical bead. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10961569-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11634754-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3889273-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113667585-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113667585-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021130495-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113025478-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113025478-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11332780-B2 |
| priorityDate |
2016-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |