| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d21e94faa5d653906fb138726aad6e63 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate |
2015-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ef578fbe2b7a30ac1989b9293716bd9a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a718c17eb5f57834c17056cab60e5d0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_481cf4225d64f54b558d54a713fa199c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fde8ac4c49e7c4cf642e9bb62b151604
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19fb435b67ce55d0c33fe4fc3de57ec1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a2e665c47db4d488ed3931d957ddf18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b1aa851c4cc07e16a616e99adac0232b |
| publicationDate |
2017-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3201350-A1 |
| titleOfInvention |
Method for nucleic acid analysis directly from an unpurified biological sample |
| abstract |
A method is provided for generating single-stranded DNA circles from a biological sample. The method comprises the steps of: treating the biological sample with an extractant to release nucleic acids, thereby forming a sample mixture; neutralizing the extractant; denaturing the released nucleic acids to generate single-stranded nucleic acids; and contacting the single-stranded nucleic acids with a ligase that is capable of template-independent, intramolecular ligation of single-stranded DNA to generate the single-stranded DNA circles. All the steps of the method are performed without any intermediate nucleic acid isolation or nucleic acid purification. The single-stranded DNA circles may be amplified and further analyzed. Also provided is a kit which comprises compositions for carrying out the novel methods. |
| priorityDate |
2014-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |