| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_675b8f22265fb4f40bdc386734e6b0fa |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y104-01002
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-03 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K4-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-57
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-805
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-01019
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0016
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0069
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y104-01002
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-03
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-01017
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-61
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-55
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-565 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-01 |
| filingDate |
2016-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_672520db5787c4bbde4ef229dfc7589c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14a0ee6a92efbfca6547b13bc6cb492d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_141668fed00847d4357211db8b5e7ca7 |
| publicationDate |
2017-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3181690-A1 |
| titleOfInvention |
Active-form mutant enzyme production method, new active-form mutant enzyme, and solubilized mutant protein production method |
| abstract |
The present invention provides a method for expressing, as a soluble protein or an active-form mutant enzyme, an enzyme that cannot be expressed as a soluble protein or an active-form enzyme in a heterologous expression system or that is obtained in a minute amount even when an active-form enzyme is expressed, the method including a technique for selecting an effective mutation site and a mutated amino acid. The present invention also provides a new active-form mutant enzyme obtained from this technique. The present invention pertains to a method for producing a soluble mutant protein or an active-form mutant enzyme and involves: specifying an insoluble protein or an inactive-form enzyme; specifying a hydrophilic amino acid that is present in a hydrophobic domain and/or a hydrophobic amino acid that is present in a hydrophilic domain of an α-helix structure portion of the insoluble protein or the inactive-form enzyme and preparing a gene that codes for an amino acid sequence in which a substitution is made to the hydrophilic amino acid that is present in the hydrophobic domain and/or the hydrophobic amino acid that is present in the hydrophilic domain, or specifying an amino acid that is relatively less preserved in terms of sequence identity in an amino acid sequence of the inactive-form enzyme, and preparing a gene that codes for an amino acid sequence in which this amino acid is substituted with an amino acid that is more preserved than the amino acid; obtaining proteins by expressing the gene in a heterologous expression system; and selecting, from the obtained proteins, a soluble mutant protein or an active-form mutant enzyme. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20190002239-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102051018-B1 |
| priorityDate |
2015-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |