http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3177931-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5f88bdedfe0cdef66997171c2416babc |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-948 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-96472 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-9513 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-988 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-924 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-23005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-23016 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54353 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54306 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01018 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y402-01001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-17021 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6804 |
| filingDate | 2015-08-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db30ee0c002d1463459a744573be35b2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f9e237e7673697d39351c7d4f7850ca http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad04ed4cc182cb4d0ee824c2089e0e75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e02f8688572ed5e409f42527125331e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2aac8f3b739c82d346a3053a585f834a |
| publicationDate | 2017-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3177931-A2 |
| titleOfInvention | Method of detection of analyte active forms and determination of the ability of substances to bind into analyte active sites |
| abstract | The invention provides a method for detection of active form of analytes in a sample and/or for determination of ability of tested substances to bind to the active site of these analytes, comprising the following steps: a) analyte or group of analytes from the sample is immobilized on the surface of a solid carrier either by non-specific non-covalent adsorption or by covalent binding of surface functional groups of the analyte and corresponding functional groups of the solid carrier, or preferably via a binding molecule which is bound to the surface of the solid carrier before immobilization of the analyte or group of analytes and is capable of selectively binding the analyte or group of analytes contained in the sample during incubation of the solid carrier with the sample; b) analyte or group of analytes is incubated with a detection probe which binds selectively to the analyte or group of analytes via a compound for selective binding to the analyte active site; whereas the probe consists of a low molecular compound for selective binding to the analyte active site; an oligonucleotide tag, optionally with a covalently attached fluorophore, biotin or a chemical group, and a chemical linker covalently linking the compound for selective binding to the analyte active site and the oligonucleotide tag; c) then the solid carrier is washed to remove unbound detection probe; and subsequently, the amount of bound detection probe is determined, whereas this amount is directly proportional to the amount of the analyte or group of analytes in the sample. The described method has broad application in medicine. Given the exceptional sensitivity of only a few dozen molecules, it provides the ability to determine the protein markers in blood in a concentration yet undetectable. |
| priorityDate | 2014-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 505.