| abstract |
An apparatus introduces a sample into a separation unit of a chromatography system with a mobile phase, including first and second mobile phase components. The apparatus includes first and second pump systems, and an injection unit. The first pump system provides the first mobile phase component, first and second portions of the first mobile phase component flowing through first and second branches, respectively. The second pump system provides the second mobile phase component, first and second portions of the second mobile phase component flowing through third and fourth branches, respectively. The injection unit receives a combined stream of the first portions of the first and second mobile phase components provided via the first and third branches, respectively, and to injects the sample into the combined stream to form a sample-containing stream. The sample-containing stream is subsequently combined with the second portions of the first and second mobile phase components provided via the second and fourth branches, respectively, to form a diluted sample-containing stream, which flows to the separation unit for separating sample constituents. |