| abstract |
Disclosed herein are methods of detecting HIV-2 nucleic acids in a sample (such as from a sample infected with or suspected to be infected with HIV-2). In some examples, the methods include LAMP or RT-LAMP, while in other examples, the methods include hybridization of a probe to an HIV-2 nucleic acid, including, but not limited to real-time PCR. Sets of LAMP primers for detection of HIV-2 Group A and Group B nucleic acids are provided herein. Sets of probes and primers for real-time PCR detection of HIV-2 nucleic acids are also provided herein. Finally, primers for amplification of HIV-2 nucleic acids are provided. Also disclosed are isolated HIV-2 nucleic acids, vectors including the HIV-2 nucleic acids, and cells transformed with vectors including HIV-2 nucleic acids. |