http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3074535-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7007295788a2c5ed87c9dad8a0bb5c95 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-125 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-131 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2014-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ac41cb88fb9a7ecca18d18dbc8df564 |
| publicationDate | 2016-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3074535-A1 |
| titleOfInvention | Rolling circle amplification method |
| abstract | The present invention is directed to a method for performing a rolling circle amplification (RCA) reaction comprising at least two rounds of RCA, said method comprising: a) providing a concatemeric first RCA product comprising a multiplicity of monomer repeats, each repeat representing a complementary copy of a first RCA template; b) cleaving the first RCA product into monomer units, wherein the monomer unit is reduced in size as compared to the monomer repeat of the first RCA product; c) circularising monomer units resulting from said cleavage to form second RCA templates wherein the second RCA template is smaller than the first RCA template; d) performing a second RCA reaction using said second RCA template of (c) and a primer for said second RCA, to form a second RCA product; wherein any one or more of steps (a) to (d) may be performed sequentially or in combination. The method may be used in the detection and/or analysis of a nucleic acid molecule, including in particular in the detection of an analyte. |
| priorityDate | 2013-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 133.