Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3b72bc0a2f48e9f1e5260dd9b0fae605 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-178 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1013 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21014 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-566 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2014-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ae7d3b3d3e8b67c18f2233c098b52bd |
publicationDate |
2016-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-3074413-A1 |
titleOfInvention |
Micro rna isolation from biological fluid |
abstract |
The disclosure provides methods and kits for isolating miRNAs from biological fluids. Specifically, the method comprising contacting a biological fluid with a surface active agent and an anti-miRNA-binding protein reagent, wherein the surface active agent dissociates biological fluid components and the anti-miRNA-binding protein reagent interacts with a miRNA-binding protein associated with miRNA to form immunoprecipitated miRNA complexes; and releasing miRNA from the immunoprecipitated miRNA complexes. Examples of miRNA-binding proteins that can be associated with circulating miRNAs are further provided. |
priorityDate |
2013-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |