| abstract |
A DNA polymerase mutant comprising SEQ ID NO:13 with a mutation at position E230 and/or E507 and optionally one or more of the following mutations E189K, H28R, L30R, L30P, G38R, F73V, H75R, E76G, E76K, E76A, E90K, K206R, E315K, A348V, L351 F, A439T, D452N, G504S, G504D, D551N, L552R, 1553V, D578N, H676R, Q680R, D732G, E734G, E734K, F749V; nwherein the polymerase mutant exhibits relative to wild-type DNA polymerase of SEQ ID NO: 13, and under the same conditions, increased polymerase speed and at least one of increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor. |