| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_60d2134a44f2b4765a9a1a36828c1d8c |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-0019
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-51
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-14 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-51
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-96
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P3-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y403-01024
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-0019 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P3-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-51 |
| filingDate |
2011-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_114657f5364953d815ec07bd7f061715
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c55f6213907b57107854a3e4efd3eaa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90478625d5a4cf5bdd4961505132a4c8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_646c21166563cb4a0040619ffd0a5387
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2444bf7a5f9ff8f15be154db768e86c5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e4273e32f8b36ec15fb5fb4acc4253c2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_82e16482b873267f9646a13f09af6e93
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_192b46feae948c3dc863e61726ee0461
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_33af892118c3c8e58d4231e3b2105fa9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3195c5ff812edc9853258745ac08ec67
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_684cc42059f2bcb5752d30e09922e208 |
| publicationDate |
2016-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3025728-A1 |
| titleOfInvention |
Method for purifying prokaryotic phenylalanine ammonia-lyase variants |
| abstract |
The present invention provides a method for purifying an Anabaena variabilis phenylalanine ammonia-lyase (AvPAL) variant with minimal aggregation. The method comprises the steps of (a) lysing bacterial cells containing the AvPAL variant by homogenization to generate a cell lysate; (b) heating the cell lysate to 65°C for 30 to 120 minutes; (c) centrifuging the heated cell lysate, wherein a supernatant comprising the AvPAL variant is retained; (d) filtering the supernatant to remove precipitates; and (e) separating the AvPAL variant from contaminating proteins by sequential chromatography over an anion exchange (AIEX) column followed by a hydrophobic interaction (HIC) column, wherein the eluate from the HIC column comprises the AvPAL variant, wherein the cysteine residues at positions 503 and 565 of said AvPAL variant have been substituted by serine residues (SEQ ID NO:11). |
| priorityDate |
2010-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |