Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bcb146c68b67089013b4c7a614504254 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y113-12007 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K49-0013 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K49-0045 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0069 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0275 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K49-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 |
filingDate |
2011-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c3332ef7ab73b1da2e318bdb4601522a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e88cdc2afcac68b6113e033b1a09a2e2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d72528595f742579f6070aba29844f71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4504cadd1ab51f9fceac29e57848fefd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e0af1fac63f7207dc7d23a2b1bd6952b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4339481e9dc89b379a20ed80215b0d79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_391729dace7ed01f7ce5f519aff58c61 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_772a499828606c2fe45bc752751fc621 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c08c65a6474ab3aa882910f72298d693 |
publicationDate |
2016-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-2990479-A1 |
titleOfInvention |
Mutant protease biosensors with enhanced detection characteristics |
abstract |
The invention relates to methods of detecting the presence or activity of a target molecule in a sample comprising a) contacting the sample with i) a polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase, the modified circularly-permuted thermostable luciferase modified relative to a parental circularly-permuted thermostable luciferase, the linker comprising a sensor region capable of interacting with a target molecule in a cell, and wherein the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to at least one of: A) the parental circularly-permuted thermostable luciferase in the presence of the target molecule, or B) the modified circularly-permuted thermostable luciferase in the absence of the target molecule; and ii) a substrate for the modified thermostable luciferase; and b) detecting luminescence in the sample. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20190106663-A |
priorityDate |
2010-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |