Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3c391e94dfb92577a3d1f3d2a7d2495e |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01021 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-035 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01004 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01091 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2437 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2445 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-625 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1037 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate |
2014-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2135095ddaacd937c0fd9a5d07513566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc45e16f644276d504afaa8c1890243c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_269cf38df3d330674645b317f89c837a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_060f421c9ce50420a40903688ee294a5 |
publicationDate |
2016-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-2970953-A1 |
titleOfInvention |
Improved surface display of functional proteins in a broad range of gram negative bacteria |
abstract |
The present invention relates to a method for the surface display of a recombinant polypeptide on the surface of a host cell, said method comprising the steps: (a) providing a host cell transformed with a nucleic acid fusion operatively linked with an expression control sequence, said nucleic acid fusion comprising: (i) a portion encoding a signal peptide, (ii) a portion encoding the recombinant polypeptide to be displayed, (iii) a portion encoding a transmembrane linker, and (iv) a portion encoding the transporter domain of an EhaA protein, and (b) culturing the host cell under conditions wherein the nucleic acid fusion is expressed and the expression product comprising the recombinant polypeptide is displayed on the surface of the host cell. |
priorityDate |
2013-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |