http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2926135-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_88534e7272a630633c9752d76a6a6f51 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-70514 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-70517 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5091 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56972 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 2013-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_24d185af9de308426b18b21671a603a5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_37729b6d22fabce1546bdb856a5a7901 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3843c75149b41b52b92048f8a3e0536a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_255b7c92dc26e47cc1944ac72c31b215 |
| publicationDate | 2015-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2926135-A1 |
| titleOfInvention | Method for determining compatibility between a donor and a recipient by means of the flow cytometric detection of alloreactive t cells |
| abstract | The invention relates to a method for determining compatibility between a donor and a recipient by means of the flow cytometric detection of alloreactive T cells, comprising the following steps: a) adding a label to a blood sample taken from a donor/recipient pair to be analyzed, referred to as the labeled sample below, wherein the label is suitable for differentiation from a sample from the recipient; the other blood sample taken from the donor/recipient pair to be analyzed is referred to as the unlabeled sample below, b) thinning the two samples at least one time with a first medium, removing cellular constituents of the samples from the liquid phase, c) separately adding back the cellular constituents, in particular in the same volume of the blood sample remainder of the labeled sample and the blood sample remainder of the unlabeled sample, in a second medium, d) mixing a portion from the unlabeled sample and a portion of the labeled sample, in particular at a ratio of approximately 1:1, in order to obtain a mixture, e) stimulating antigen-presenting cells (APCs) in the mixture of labeled and unlabeled sample as per step d) of the claim by adding anti-CD28 or anti-CD28 and anti-CD49d antibodies followed by f) incubating the mixture for the first time for a duration of 1.5 h to 2.5 h, preferably 2 h, at a temperature of 35-39°C, preferably 37°C, optionally under a protective-gas atmosphere, CO2 atmosphere, g) adding a secretion inhibitor, h) mixing the sample from step d) of claim 1, i) followed by incubating for a second time for a duration of at least 2.5 h at a temperature of 35-39°C, preferably 37°C, optionally under a protective-gas atmosphere, CO2 atmosphere, j) optionally lysing erythrocytes, k) detecting lymphocytes by flow cytometry by means of the label and detecting CD4 or CD8 lymphocytes by flow cytometry, for example by means of labeled anti-CD4 and/or anti-CD8 antibodies, l) detecting lymphocytes activated by means of an intracellular cytokine and lymphocytes expressing an activation marker by flow cytometry, m) wherein alloreactivity between the individual from whom the labeled sample was taken and the individual from whom the unlabeled sample was taken is established if the following results occur in the flow cytometric detection: a. label pos/CD4 pos/DC69 pos/INFgamma pos, b. label neg/CD4 pos/CD69 pos/INFgamma pos, c. label pos/CD8 pos/CD69 pos/INFgamma pos, d. label neg/CD8 pos/CD69 pos/INFgamma pos. |
| priorityDate | 2012-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.