| abstract |
The invention provides a method for detecting fluorescence radiation from a fluorescence agent, the method comprising -emitting light at an excitation wavelength range (72) for causing fluorescence radiation emission in the fluorescence agent, said fluorescence radiation having a fluorescence wavelength profile (73); -detecting light at a first fluorescence wavelength range (74) as a first detection signal (S1); -detecting light at a second fluorescence wavelength range (81, 91) as a second detection signal (S2); -numerically determining a third detection signal with an improved fluorescence-to- background radiation ratio based on the first detection signal (S1), the second detection signal (S2), and the fluorescence wavelength profile (73). |