abstract |
Described herein are compositions, methods, a system, and kits for detection of endocrine disruptor chemicals (EDCs) in environmental and other samples, such as samples of water including but not limited to waste water treatment plant effluent, using a live-cell fluorescence-based nuclear translocation reporter system. Upon binding of a ligand to a fluorescent-labeled reporter protein, the protein (and therefore the fluorescence) is translocated in a ligand level-dependent manner from the cytoplasm to the nucleus of live mammalian cells; this translocation is detectable as diffuse (cytoplasmic) fluorescence converting to localized, brightly fluorescent nuclei. The described methods, kits and system can be used to reliably detect very low levels of EDC contamination, including in high throughput analysis systems as described. |