http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2841598-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d76f7265995ded06b66b9bbf740d3f2c |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2013-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_72153e7e036cf98e4d16e2b0afc0abfa |
| publicationDate | 2015-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2841598-A1 |
| titleOfInvention | Method for competitive allele-specific cdna synthesis and differential amplification of the cdna products |
| abstract | The present invention provides a method for the detection of the presence of RNA variants comprising the step of performing a competitive c DNA synthesis comprising a first primer specific to a first RNA variant, a second primer specific to a second RNA variant, an RNA-dependent DNA polymerase, and RNA from said sample as a template, wherein said first primer and said second primer comprise an allele-specific nucleotide portion, a target-specific sequence and tag units with a common sequence and/or a discriminating sequence so that the sequence of said tag units is not complementary to said first or second RNA variant, wherein each of the c DNA products obtained from said competitive c DNA synthesis consists of the sequence of only one primer extended by the sequence complementary to one of the target RNA variants. |
| priorityDate | 2012-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 125.