| abstract |
A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a non¬ aqueous solvent, and coupling the biomolecule complex to a sub¬ strate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immo¬ bilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions. |