| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3244ba8390b1f290f4ba36389c82488b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-04 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2012-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d41f261a10c61ac92ae7e93cfed0268f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f7837fcf36f642464c45b415c2e4a7e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d65477201d15be7a482cf7f6258a173 |
| publicationDate |
2014-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-2794916-A1 |
| titleOfInvention |
Methods and reagents for reducing non-specific amplification |
| abstract |
The present invention provides methods and reagents for use in the amplification of nucleic acids. Amplification and detection carried out using probe oligonucleotides containing an N2-benzyl-dG nucleotide can result in less non-specific amplification involving the probe compared to amplification carried out using unmodified oligonucleotides, presumably because the presence of a bulky N2-benzyl-dG minor groove binder in a template prevents extension of the complementary strand by a DNA polymerase. |
| priorityDate |
2011-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |