Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_32cce36e3f60c81140719da5fa69d423 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2513-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-33 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0226 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0231 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0242 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0655 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 |
filingDate |
2012-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f67c750d8310e9bd0503e5ca18d9eea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93e7b1990bf29e1930d98d893be765e0 |
publicationDate |
2014-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-2787074-A1 |
titleOfInvention |
Method for long-term storage of porous body bearing chondrocytes |
abstract |
Disclosed is a method for storing a cartilage-cell-adhering porous body in an airtight state over a long term of at shortest 14 days. The method comprises suspending isolated cartilage cells into atelocollagen at an administration cell concentration of 1.0 × 10 7 to 1.0 × 10 8 cells/ml to prepare a cell suspension, seeding the cell suspension to a biocompatible porous body, gelatinizing the cell suspension on the porous body to yield the cartilage-cell-adhering porous body, and keeping the cartilage-cell-adhering porous body at a temperature of 26 to 37°C in a medium having an amount of 1 mL or more per 1.0 × 10 5 cells that are administrated living cells out of the cartilage cells, containing insulin, a fibroblast growth factor, and a 5% or more serum, while the porous body is shaken to set the shaking speed of the medium in the range of 0 to 2.8 cm/s. |
priorityDate |
2011-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |