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publicationDate 2014-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-2775291-A1
titleOfInvention Apparatus for differentiating multiple fluorescence signals by excitation wavelength
abstract An instrument includes: a sample well (10), a sample (12) arranged in the sample well, a light source (16), a detector (18), and a lightpipe (24) arranged between the light source and the sample. The sample includes a plurality of components and a plurality of detectable markers. Each of the plurality of detectable markers is capable of generating increased emission due to a presence of a target component in the sample and has a respective excitation wavelength range and a respective emission wavelength range. The light source is capable of emitting a plurality of excitation wavelength ranges toward the sample to respectively excite each of the detectable markers. Each of the plurality of excitation wavelength ranges corresponds to a respective excitation wavelength range of a respective one of the plurality of detectable markers. The detector is capable of detecting an emission wavelength range emitted from each of the plurality of detectable markers.
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