http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2772552-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5bc5292a07e34d16eb1d033a16d3aec3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4784a8f3d50f717860a6236f807c1764 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D417-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D413-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09B23-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D279-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09B23-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2003-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e54e17d286857907da3a0e8b47dfbc51 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54814476d976ce39ad34ae8c5a1c06e5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ec2c1fd5029af126eb9e7071c259025 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2cb025da7421ecbb7ce117b0d93b5e5c |
| publicationDate | 2014-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2772552-A1 |
| titleOfInvention | Amplicon melting analysis with saturation dyes |
| abstract | The invention provides: nA kit for amplifying and subsequently melting a plurality of target nucleic acids comprising: n(a) amplification reagents; n(b) a dsDNA binding dye having a percent saturation of at least about 90%; n(c) information on amplifying each of the plurality of target nucleic acids in the presence of the dsDNA binding dye using the amplification reagents, to generate a plurality of amplified nucleic acids; and n(d) information on generating a fluorescence melting curve from each of the plurality of amplified nucleic acids and plotting the difference between each of the fluorescence melting curves. n The invention also provides a method of PCR analysis comprising the steps of: nproviding replicates of a mixture of a dsDNA binding dye, a target nucleic acid having a specific genotype, and primers configured for amplifying the target nucleic acid, namplifying each of the replicates of the target nucleic acid in the presence of the dsDNA binding dye, nmonitoring fluorescence of the dsDNA binding dye, generating a melting curve for each of the replicates of the target nucleic acid, nrepeating the providing, amplifying and generating steps with at least one additional target nucleic acid, nestablishing the target nucleic acid melting curve as a standard across temperatures using the replicates of the target nucleic acid having the specific genotype, and nplotting a fluorescence difference between the standard and the melting curve of the at least one additional target nucleic acid. |
| priorityDate | 2002-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 528.