http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2767834-A2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_49f9482d0ae56f3c77198101cae760a9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6239ff6fe13e938cdf6854d9e1d1dee7 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07B2200-05 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-705 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K19-00 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
filingDate | 2012-04-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57fa05a63740db8475e251ab555b9063 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f7e91957c5a551dd0a8a6ee793e3ef0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e1bb8ab31f07403b8aeb75abcf463f4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a24cae78b567de51ee6f47e4b9d3e57e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_76fa1ef92398fb5f4e53c878fa32bdd4 |
publicationDate | 2014-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-2767834-A2 |
titleOfInvention | Quantitative standard for mass spectrometry of proteins |
abstract | The present invention provides a method of determining the absolute amount of a target polypeptide in a sample, said method comprising the following steps: (a) adding (aa) a fusion polypeptide to said sample, said fusion polypeptide comprising (i) at least one tag sequence and (ii) a subsequence of the target polypeptide; and (ab) a known absolute amount of a tag polypeptide comprising or consisting of said tag sequence according to (aa) to said sample, wherein said fusion polypeptide on the one hand is mass-altered as compared to said target polypeptide and said tag polypeptide on the other hand, for example, said fusion polypeptide on the one hand and said target polypeptide and said tag polypeptide on the other hand are differently isotope labeled; (b) performing proteolytic digestion of the mixture obtained in step (a); (c) subjecting the result of proteolytic digestion of step (b), optionally after chromatography, to mass spectrometric analysis; and (d) determining the absolute amount of said target polypeptide from (i) the peak intensities in the mass spectrum acquired in step (c) of said fusion polypeptide, said tag polypeptide and said target polypeptide and (ii) said known absolute amount of said tag polypeptide. n Furthermore provided is a fusion polypeptide for the quantification of a target polypeptide by mass spectroscopy, wherein: said fusion polypeptide consists of 35 to 455 amino acid residues and comprises (i) a target region, which is a fragment of the target polypeptide, and (ii) a tag region, which is not a fragment of the target polypeptide, said target region consists of 15 to 205 amino acid residues and comprises at least two signature regions; said tag region consists of 20 to 250 amino acid residues and comprises at least two signature regions; and each signature region has the structure Y-Z-X 4-28 -Y-Z, wherein all Y:s are selected from one of (i)-(iv), wherein (i) is R or K, (ii) is Y, F, W or L, (iii) is E and (iv) is D and each X and each Z are independently any amino acid residue, provided that the Z:s are not P if the Y:s are selected from (i)-(iii); and each signature region comprises at least one amino acid residue comprising a heavy isotope. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11264117-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11183286-B2 |
priorityDate | 2011-04-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 68.