http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2756079-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efda7341819f1a85d7f2fe104cd8a26f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2011-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04014980e809ce52a32cabe8a3addd89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35644269bd40774244ce2c3eafb173b7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d45b0e68f5bda3167be133e94cbfa024 |
| publicationDate | 2014-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2756079-A1 |
| titleOfInvention | Method for isolating nucleic acids from a veterinary whole blood sample |
| abstract | The present invention provides a method for isolating nucleic acids from a veterinary whole blood sample, said method comprising at least the following steps a) preparing a binding mixture comprising - the lysed sample - at least one chaotropic agent - at least one alcohol - at least one polyoxyethylene fatty alcohol ether; b) passing the binding mixture through a column comprising a nucleic acid binding solid phase thereby binding the nucleic acids to the nucleic acid binding solid phase; c) optionally washing the nucleic acids while being bound to the solid phase; d) optionally eluting the nucleic acids from the solid phase. It was found that the addition of the specific non-ionic detergent overcomes the problems of the prior art methods, wherein the column clogs what prevents the efficient nucleic acid isolation from this difficult sample. When the specific non-ionic detergent is included into the binding mixture, no clogging occurs thereby allowing the efficient isolation of nucleic acids from veterinary whole blood samples. |
| priorityDate | 2011-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 181.