http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2743354-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e9e4bfa6b7131aa040cdf49cdb20b883 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2012-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd69932c8ec23aade84fea580d99fca6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e1f77d992ef2550a2e68f0b73e567bd4 |
| publicationDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2743354-A1 |
| titleOfInvention | Low melting temperature primer |
| abstract | The present invention relates to a polynucleotide comprising a combination of at least two modifications selected from the group of a d-spacer (abasic site), a short linker, an unlocked nucleic acid, a glycol nucleic acid, a triazole linker and an introduced additional negative charge. n The invention further relates to a method for isothermal amplification of a nucleic acid comprising the steps of (a) combining in a reaction mixture one or more template nucleic acids, an enzyme with amplification activity, at least one polynucleotide comprising a combination of at least one modification selected from the group of a d-spacer (abasic site), a short linker, an unlocked nucleic acid, a glycol nucleic acid, a triazole linker and an introduced additional negative charge, optionally one or more unmodified polynucleotides, a buffer, and deoxynucleotide triphosphates and (b) performing an amplification reaction. |
| priorityDate | 2012-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 217.