| abstract |
A method for producing ectoine or a derivative thereof comprises the steps of (a) preparing host cells from a type of yeast, the host cells containing at least one recombinant DNA sequence encoding each of one or more heterologous enzymes for biosynthesis of ectoine, (b) causing the host cells, with the aid of the recombinant DNA sequences encoding the enzymes, to express the heterologous enzymes, and (c) causing the host cells, with the aid of the expressed heterologous enzymes, to synthesize ectoine and/or the derivative thereof from a precursor. A yeast cell of the orders Saccharomycetales or Schizosaccharomycetales for use as a host cell in such a method contains at least one recombinant DNA sequence encoding each of one or more heterologous enzymes for biosynthesis of ectoine. |